Biotechnology
Zinah A. Ghareeb; Hiba Abdel-Hassan AlKhafaji; Mohanad K. Aneed Al-Saedi
Abstract
Familial hypercholesterolemia (FH) is a genetic disorder characterized by autosomal inheritance in genes related to LDL-C metabolism, with the major clinical features of hyper-LDL-cholesterolemia and premature coronary artery disease. (LRP-1) is a member of the LDLR family. It is a membrane receptor ...
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Familial hypercholesterolemia (FH) is a genetic disorder characterized by autosomal inheritance in genes related to LDL-C metabolism, with the major clinical features of hyper-LDL-cholesterolemia and premature coronary artery disease. (LRP-1) is a member of the LDLR family. It is a membrane receptor with scavenging and signaling properties. LRP-1 interacts with a wide range of extracellular ligands as well as intracellular scaffolding and signaling proteins, which makes it important in crucial clinical circumstances like cardiovascular disease, cancer, and neurological illnesses. Mir-205 uses these molecules as biomarker for cardiovascular diseases. This study aims to measure gene expression for the LPR-1 gene and its relationship to the development of cardiovascular disease in familial hypercholesterolemia and non-familial hypercholesterolemia. Also, it studies the indication whether mir-205 regulates the action of the LRP-1 gene in terms of increasing or decreasing gene expression. However, the available methods for measuring LRP1 levels are direct and quantitative using Poly Chain Reaction (RT-PCR) in real time, not at its ends. In the present study, blood was isolated from 150 individuals distributed into three groups: Group 1 included: 50 samples from a healthy group; Group 2: 50 samples from non-Familial hypercholesterolemia patients; Group 3:50 samples Familial hyperchol-esterolemia patients. The results showed that LRP1 protein expression was significantly reduced in patients with F.H compared with normal control in a small cohort from an Iraqi population. This pilot study suggests that the reduced LRP1 protein expression may be associated with cardiovascular disease progression.
Biotechnology
Media F. Ali Jan; Basima Q. Hassan Al-Saadi; Hiba M. Abdel-Hassan Al-Khafaji; Mohanad K. Aneed Al-Saedi
Abstract
Asthma is one of the most prevalent non-communicable illnesses, and it has a significant influence on the quality of life for many people. Asthma is now thought to be a multifaceted condition, with interactions between genetic susceptibility, host factors, and environmental exposures increasingly being ...
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Asthma is one of the most prevalent non-communicable illnesses, and it has a significant influence on the quality of life for many people. Asthma is now thought to be a multifaceted condition, with interactions between genetic susceptibility, host factors, and environmental exposures increasingly being blamed for its pathogenesis. In respiratory illness, interleukin-8 plays a critical function due to promoting phagocytosis and inducing angiogenesis. Also, identification and finding out the effect of these SNPs and how it can be dangerously related or contribute to a Bronchial Asthma disease. However, the available methods for detecting interleukin-8 gene polymorphism are direct and quantitative using Poly Chain Reaction (RT-PCR) in real time. Whole blood was isolated from 100 patients with asthma and 50 healthy individuals. The results of polymorphism in single nucleotides showed an essential role in the development of asthma and that the presence of these SNPs has a role in increasing the susceptibility of individuals to asthma, as the variation in the TT genotype at the site followed by the AT genotype at the same site shows high-risk damage.