Laser Science and Technology
Kareem H. Jawad; Butheina Hasson
Abstract
Porous silicon nanoparticles, or PSNPs, are one of the most common NPS with distinct characteristics. PSNPs were created using an enhanced approach known as the electrochemical etching process. The nanoparticles were treated with a post-laser to obtain the nanoparticles, which were subsequently analyzed ...
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Porous silicon nanoparticles, or PSNPs, are one of the most common NPS with distinct characteristics. PSNPs were created using an enhanced approach known as the electrochemical etching process. The nanoparticles were treated with a post-laser to obtain the nanoparticles, which were subsequently analyzed using (SEM), (UV-Vis), and (XRD). The size refers to porous silicon with a nanostructure. Because of the differences in interaction between the HF electrolyzed and silicon and crystal structure, PS (100) offers better NP properties than directional silicon (111). PSNPs' antioxidant activity was measured using the DPPH test, while cytotoxicity was measured using the MTT assay on Hella cells. PSNPs have an inhibitory impact on cancer cell growth and antioxidants, according to the findings. PSNPs did not have any toxicological effects on the skin, lungs, or spleen after injection.
Biotechnology
Butheina Hasson; Likaa Mahdi; Rajwa Essa
Abstract
Probiotic strains have the potential to be used as bio-preservatives and functional radical scavenging treatments in the future. Antioxidant tests, including DPPH radical scavenging, were used to evaluate the antioxidant effects of extracellular L- Glutaminase isolated from L. gasseri BRLHM. Parameters ...
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Probiotic strains have the potential to be used as bio-preservatives and functional radical scavenging treatments in the future. Antioxidant tests, including DPPH radical scavenging, were used to evaluate the antioxidant effects of extracellular L- Glutaminase isolated from L. gasseri BRLHM. Parameters for the promoted production of the enzyme under minimal production media were optimized. The importance of this study lies in enhancing the production of the L- Glutaminase isolated from L. gasseri BRLHM with a high activity using these L. gasseri bacterial as antioxidant activity. In ion-exchange chromatography, the specific activity was 14.7U/mg protein, with 58.8% yield and 4.6 purification folds. In the gel filtration, the specific activity was 23.4 U/mg protein, with a yield of 54 % and 4.6 purification folds. According to the findings, L-Glutaminase isolated from L .gasseri BRLHM exhibited good antioxidant properties. As the concentration rose, there was a remarkable proportionate increase in scavenging activity. The IC50 values for control and L- Glutaminase were 36.09 1.12 and 619.8 gm/ml, respectively. The IC50 values were discovered to be 100 and 200 μg/ml, respectively. Conclusion: For the first time, the high of L- Glutaminase isolated L. gasseri BRLHM was shown to exhibit antioxidant. This could be a promising discovery for future radical scavenging treatments and antioxidant prophylaxis with natural probiotics.
