Biotechnology
Halah F. Rafeeq; Zahraa A. Sharba
Abstract
Klebsiella pneumoniae is a noteworthy human pathogen. As a virulence factor, these bacteria may create a thick coating of extracellular biofilm. This aids the organism's adhesion to biotic as well as abiotic surfaces, preventing antimicrobial agents from doing their job. Infections caused by bacterial ...
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Klebsiella pneumoniae is a noteworthy human pathogen. As a virulence factor, these bacteria may create a thick coating of extracellular biofilm. This aids the organism's adhesion to biotic as well as abiotic surfaces, preventing antimicrobial agents from doing their job. Infections caused by bacterial biofilms have become more difficult to treat as a result. Therefore, the present study has been designed to investigate the effects of essential oils, individually or in combination, on the biofilms of Klebsiella pneumoniae isolates. In this research, the quantification of biofilm composition for 50 isolates from urine samples indicates the following statistics: [n = 24 (48%)] isolates form a strong biofilm, [n = 12 (24%)] a moderate biofilm, [n = 10 (20%)] a weak biofilm, and [n = 4 (8%] a non-biofilm. The Minimum Inhibitor Concentration (MIC) and MBC values for essential oils were determined. The results showed that the MIC for tea tree oil was 0.25% and MBC 0.5%. While the MIC for cinnamon oil was 0.125%, and MBC was 0.25%. Afterward, the anti-biofilm effectiveness of essential oils was evaluated. The results showed that both oils had good efficacy against strong biofilm for Klebsiella pneumoniae isolates. But in a comparison between them, cinnamon oil showed better results. Due to the efficacy of these two oils, the combined impact of the two oils was discovered in this study. And the results revealed that there was an antagonistic effect. These findings recommend additional essential oils be tested to see how they affect biofilms of Klebsiella pneumoniae or other bacteria.
Biotechnology
Noor Younus; Maha Altaee; Zahraa Abdul-Munim Sharba
Abstract
This study was carried out to describe the gene expression of the micro RNA 122a gene with the development of diabetes in Iraq. The difference in gene expression between patients and healthy controls was properly considered. In this study, blood was isolated from 121 individuals divided into two groups ...
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This study was carried out to describe the gene expression of the micro RNA 122a gene with the development of diabetes in Iraq. The difference in gene expression between patients and healthy controls was properly considered. In this study, blood was isolated from 121 individuals divided into two groups as follows: 80 samples of diabetic patients and 41 samples from a healthy control. miRNA was isolated and transformed into cDNA, and the expression of mi122a was measured by qRT-PCR. The researchers looked at the relationship between age and gender and the occurrence of diabetes, as well as how they compared to controls. When comparing the mean gene expression level (Ct) of patient groups to the corresponding Ct means in the control group, the results revealed a discrepancy. Also the gene expression folding (2-∆∆Ct) of the micro RNA 122a gene reflect differences in the expression, the level of micro RNA 122a was (20.504) in patients with diabetes compared to control groups with significant differences. On the other hand, gender and family history showed a significant difference between patients and health monitors. For age and type of diabetes, they showed a significant difference between patients and health monitors. Our results indicate that diabetes can affect all ages in both males and females. This study aims to correlate the expression of miRNA 122a with the occurrence of diabetes in the Iraqi population.
