Online ISSN: 2788-6867

Keywords : L-glutaminases

Evidence of Antioxidant Activity of Novel L-Glutaminase Purified from L. Gasseri BRLHM

Butheina Hasson; Likaa Mahdi; Rajwa Essa

Journal of Applied Sciences and Nanotechnology, 2021, Volume 1, Issue 4, Pages 44-51
DOI: 10.53293/jasn.2021.3969.1064

Probiotic strains have the potential to be used as bio-preservatives and functional radical scavenging treatments in the future.  Antioxidant tests, including DPPH radical scavenging, were used to evaluate the antioxidant effects of extracellular L- Glutaminase isolated from L. gasseri BRLHM. Parameters for the promoted production of the enzyme under minimal production media were optimized.    The importance of this study lies in enhancing the production of the L- Glutaminase isolated from L. gasseri BRLHM with a high activity using these L. gasseri bacterial as antioxidant activity. In ion-exchange chromatography, the specific activity was 14.7U/mg protein, with 58.8% yield and 4.6 purification folds.   In the gel filtration, the specific activity was 23.4 U/mg protein, with a yield of 54 % and 4.6 purification folds. According to the findings, L-Glutaminase isolated from L .gasseri BRLHM exhibited good antioxidant properties. As the concentration rose, there was a remarkable proportionate increase in scavenging activity. The IC50 values for control and L- Glutaminase were 36.09 1.12 and 619.8 gm/ml, respectively. The IC50 values were discovered to be 100 and 200 μg/ml, respectively. Conclusion: For the first time, the high of L- Glutaminase isolated  L. gasseri BRLHM was shown to exhibit antioxidant. This could be a promising discovery for future radical scavenging treatments and antioxidant prophylaxis with natural probiotics.