Journal of Applied Sciences and Nanotechnology

Aluminum oxide, often known as Al₂O₃, is a chemical compound of aluminum and oxygen with the formula Al₂O₃. It's the most common of many aluminum oxides, known as aluminum (III) oxide. The study investigates the cytotoxicity and antibacterial effects of Aluminum oxide nanoparticles (Al₂O₃-NPs) in different cells and bacteria. Different characterization methods such as dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) have been used to evaluate morphologies and physicochemical properties of Al₂O₃-NPs. MTT technique is used for determining NPs cytotoxicity. The size distribution of Al₂O₃-NPs was 68 ± 12 nm in diameter, while the zeta potential was (-36 ± 10 mV). There is no toxicity by using the MTT assay, as well as showed antibacterial activity was formed at 200 µg/mL, while the higher antibacterial activity was occurring at (18 ± 0.2) and (17 ± 0.1) for Proteus Vulgaris and Streptococcus pyogenes, respectively. The findings confirmed that the Al₂O₃-NPs have small dimensions, high stability, and increased antibacterial activity.