Journal of Applied Sciences and Nanotechnology

Biosynthesis of AgNPs is a new approach in the field of nanotechnology with optimistic implementation in medicine, food control, and pharmacology. In this study, the silver nanoparticles were produced by Lactobacillus gasseri filtrate. The production of AgNPs was confirmed by the color change from yellow to brown. Using UV visible spectrophotometer at 424 nm wavelength, and X-ray diffraction, it was found that the size of the synthesized particles was 58.5 nm after applying Scherrer’s equation. The inhibitory activity of silver nitrate on the growth of some pathogenic isolates was studied Staphylococcus haemolyticus Gram positive, and Klebsiella pneumoniae Gram negative. The highest inhibitory diameter was 14.6 mm at 100% concentration (stock) against Staphylococcus haemolyticus bacteria was followed by that of Klebsiella pneumoniae bacteria with an average inhibition zone diameter reached 13.6 mm at 100% concentration. The highest effect was of AgNPs on the growth of Staphylococcus haemolyticus bacteria, as it found the average diameter of the inhibition zone reached to 29.3 mm, followed by Klebsiella pneumoniae with the average diameter of the inhibiting zone it was 22.6 mm at 100% concentration (stock). This study showed AgNPs have more antibacterial activity against Gram positive bacteria than Gram negative bacteria. The importance of this study lies in testing the effectiveness of by Lactobacillus gasseri bacteria in the biosynthesis of silver nanoparticles and studying their antibacterial activity on pathogenic bacteria.

Drug delivery using nanocarriers is recommended to decrease the drug amount. To improve the different therapeutic characteristics of curcumin (CU) such as solubility, bioavailability, maintenance endorsement, and make it a promising, successful antitumor drug used for prostate cancer treatment. It was introduced to folate decorated chitosan (CS) coated Fe@Au NPs (FA-CU-CS-Fe@Au NPs). Fe@Au nanoparticle contains magnetic Fe NP’s core with a fine layer of Au NP’s synthesized using the method Pulsed, Laser, Ablation in Liquid (PLAL). These Fe@Au NP’s characterized by UV-Visible Spectrophotometer, High-Resolution, Transmission Electron Microscopy, (HRTEM), and Field Emission Scanning, Electron, Microscopy (FESEM). The smallest nanosize and the best result was obtained at different laser wavelength (532, 1064) nm. The mean size gained of Fe@Au NPs were (67.65, 77.88) nm. Obtained results exhibited that the laser wavelength plays a key role in the size, and dispersity of Fe@Au NPs. CU loaded FA-CS-Fe@Au NPs MTT assay on human prostate cancer cell line (PC3) proved that CU cytotoxicity can improve when they are loaded on (FA-CS-Fe@Au NPs) when comparing it with free CU.