Nanotechnology
Mohammad Reza Hosseini VeleshKolaei; Pooria Gill; Adele Rafati; Marzieh Adiani
Abstract
Considering the introduction of aptamers as a new generation of analyte identifiers, this class of materials can be used in diagnostic systems because aptamers are easier to produce, more sensitive, higher accuracy, less sensitive to environmental factors, easier to handle and can be used. A particular ...
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Considering the introduction of aptamers as a new generation of analyte identifiers, this class of materials can be used in diagnostic systems because aptamers are easier to produce, more sensitive, higher accuracy, less sensitive to environmental factors, easier to handle and can be used. A particular type of aptamer that has a sequence rich in guanine base can create a unique nanostructure called G-quadruplex. The creation of this structure gives the aptamer an enzyme property so that it can act like an enzyme in the vicinity of it, oxidise a chromogenic substrate and produce a coloured signal. The main way to produce aptamers is a laboratory technique called SELEX (Systematic evolution of ligands by exponential enrichment), in which a mixture of different oligo libraries near the target analyte creates aptamers in several consecutive cycles. The aim of this study was to introduce a novel approach for obtaining DNA aptamers for detecting a ligand such as an aflatoxin M1 in bioinformatically manner in replacing SELEX for obtaining the specific oligo aptamers against aflatoxin M1. For this purpose, the selected oligoaptamers' structures were predicted using molecular simulators and bioinformatic techniques. The results of these molecular simulations suggested G-quadruplex aptamers with a suitable affinity for binding to aflatoxin M1 in colourimetric assays.
Biotechnology
Abdalrahman S. Mohammed; Abbas A. Al-Janabi
Abstract
In the current study, bioinformatics approach has been utilized to discover the sequences and structures analysis of IL-17A of breast cancer and compares with normal sequence from NCBI. The current study was aimed to discuss the possibility of using IL-17A as a marker for patients with breast cancer. ...
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In the current study, bioinformatics approach has been utilized to discover the sequences and structures analysis of IL-17A of breast cancer and compares with normal sequence from NCBI. The current study was aimed to discuss the possibility of using IL-17A as a marker for patients with breast cancer. Also, the effect of mutation on the physicochemical properties and structure of IL-17A. Sixty blood samples were examined from patients with breast cancer (aged between 20 and 75 years old). In patients with breast cancer, there were missense mutations and deletion mutations detected by BLAST. Furthermore, the current study determined the physicochemical properties of IL-17A, such as their hydrophilic nature; alpha–helical structure, and 3D structure. The results of this study show that IL-17A consider as a marker for the patient with breast cancer. Also, the mutations on IL-17A gene affected the structure and physicochemical properties of the Interleukin-17A protein complex.
Biotechnology
Batool K. Queen; Abbas A. Mohammed
Abstract
In this paper, the study aimed to analyze the sequence and structure of Transcription Factor 7 Like 2 gene (TCF7L2) in Iraqi Diabetic Mellitus Type II (T2DM) Patients and was compared with standard sequence form National Center of Bioinformatics Tools (NCBI) using BLAST. Ten blood samples of (T2DM) Iraqi ...
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In this paper, the study aimed to analyze the sequence and structure of Transcription Factor 7 Like 2 gene (TCF7L2) in Iraqi Diabetic Mellitus Type II (T2DM) Patients and was compared with standard sequence form National Center of Bioinformatics Tools (NCBI) using BLAST. Ten blood samples of (T2DM) Iraqi patients, was collected from Al-Mustansiriya University National Diabetes Centre in Baghdad Province/Iraq (17-65 year). DNA was extracted from whole blood of patients’ samples using the Quick-DNA™ Blood MiniPrep kit, then it was sent to Korea at Macrogen Corporartion Company, where they used automated DNA sequencing for sequence analysis. The results of the sequence analysis of (T2DM) patients’ samples, we found: six missense mutations, one deletion mutation, and three silent mutations detected using BLAST in (NCBI). All mutations appeared at the same sites of the gene which controls the rate of genetic information transcription that indicates to have a relationship with (T2DM). These mutations were recorded on the (NCBI). The physicochemical properties of (TCF7L2) determined in the present study included; alpha-helical structure and 3-Dimension structure appeared contrast when compared with the gene template. Briefly, mutations effected (TCF7L2) which influences the structure, physicochemical properties of the protein, and the secretion of insulin hormone which maintains glucose level in blood.
