Journal of Applied Sciences and Nanotechnology

Abstract

Iron oxides are the most common types of substances available in nature in various forms such as oxides, hydroxides and oxide-hydroxides, which obtained from various aqueous reactions. In the present study, iron oxide (Fe₃O₄) nanoparticles (NPs) had been synthesized using co - precipitation method and determined some biological activities. Iron oxide NPs had been characterized by spectrophotometry, X- Ray Diffraction (XRD) analysis and Field Emission Scanning Electron Microscopic (FE-SEM). The XRD analysis confirmed the purity and crystalline nature of fabricated NPs. In contrast, four biological activities of iron oxide NPs were determined.  High removal of methylene blue pollutant dye (37.62 %) was observed when iron oxide NPs were used at 400 µg/ml within 48 hrs. Furthermore, iron oxide NPs revealed high inhibition zones of 27.5 and 30 mm, at 1000 µg/ml, toward Staphylococcus aureus and Escherichia coli respectively. The hemolysis activity of fabricated NPs is increases whenever concentrations increased. The lower hemolysis percentage of iron oxide NPs was (69.76%) when the NPs concentration was 250 µg/ml. Finally, cytotoxic activity was estimated against MCF- 7 cell line and normal cell line WRL68 by MTT assay. Significant and decrease in viability of (MCF-7 to 65.1%) when 400 µg/ml of iron oxide NPs was examined, while WRL68 viability was (75.03%).