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  2. Volume 1, Issue 3
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Online ISSN: 2788-6867

Volume1, Issue3

Correlation of MicroRNAs-122a Gene Expression with Diabetic for Iraqi Patients

    Noor Younus Maha Altaee Zahraa Abdul-Munim Sharba

Journal of Applied Sciences and Nanotechnology, 2021, Volume 1, Issue 3, Pages 64-72
10.53293/jasn.2021.3789.1043

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Abstract

This study was carried out to describe the gene expression of the micro RNA 122a gene with the development of diabetes in Iraq. The difference in gene expression between patients and healthy controls was properly considered. In this study, blood was isolated from 121 individuals divided into two groups as follows: 80 samples of diabetic patients and 41 samples from a healthy control. miRNA was isolated and transformed into cDNA, and the expression of mi122a was measured by qRT-PCR. The researchers looked at the relationship between age and gender and the occurrence of diabetes, as well as how they compared to controls. When comparing the mean gene expression level (Ct) of patient groups to the corresponding Ct means in the control group, the results revealed a discrepancy. Also the gene expression folding (2-∆∆Ct) of the micro RNA 122a gene reflect differences in the expression, the level of micro RNA 122a was (20.504) in patients with diabetes compared to control groups with significant differences. On the other hand, gender and family history showed a significant difference between patients and health monitors. For age and type of diabetes, they showed a significant difference between patients and health monitors. Our results indicate that diabetes can affect all ages in both males and females. This study aims to correlate the expression of miRNA 122a with the occurrence of diabetes in the Iraqi population.
Keywords:
    miR122a Diabetes Gene Expression metabolic disorders insulin
Main Subjects:
  • Biotechnology
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(2021). Correlation of MicroRNAs-122a Gene Expression with Diabetic for Iraqi Patients. Journal of Applied Sciences and Nanotechnology, 1(3), 64-72. doi: 10.53293/jasn.2021.3789.1043
Noor Younus; Maha Altaee; Zahraa Abdul-Munim Sharba. "Correlation of MicroRNAs-122a Gene Expression with Diabetic for Iraqi Patients". Journal of Applied Sciences and Nanotechnology, 1, 3, 2021, 64-72. doi: 10.53293/jasn.2021.3789.1043
(2021). 'Correlation of MicroRNAs-122a Gene Expression with Diabetic for Iraqi Patients', Journal of Applied Sciences and Nanotechnology, 1(3), pp. 64-72. doi: 10.53293/jasn.2021.3789.1043
Correlation of MicroRNAs-122a Gene Expression with Diabetic for Iraqi Patients. Journal of Applied Sciences and Nanotechnology, 2021; 1(3): 64-72. doi: 10.53293/jasn.2021.3789.1043
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Journal Information

Publisher: University of Technology - IRAQ

Email:  jasn@uotechnology.edu.iq

Editor-in-chief: Prof. Dr. Adawiya J. Haider

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Applied Sciences and Nanotechnology (JASN)

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