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Online ISSN: 2788-6867

Volume2, Issue1

Determination of Interferon Gamma Protein in Serum of Breast Cancer Patients Using the ELISA

    Noor E. Hassan Abbas A. Mohammed

Journal of Applied Sciences and Nanotechnology, 2022, Volume 2, Issue 1, Pages 37-48
10.53293/jasn.2021.4295.1089

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Abstract

Interferon gamma (IFN-γ) is a cytokine involved in the induction and modulation of various immune responses. INF-γ has recently been associated with breast cancer and the stage of the disease. Also, it can be used as a cancer marker in determining disease activity. The ELISA test is a technique for identifying antigens in biological materials. The ELISA test is used for the detection of antibodies against target antigen through highly antibody-antigen interactions. In this study, the blood samples were taken from 88 Iraqis, 30 healthy controls, and 58 breast cancer patients (ranging from 35 to 52 years of age). During a visit to the AL Amal National Hospital for Cancer Treatment in Baghdad, Iraq, these patient samples were taken October 2020 - January 2021. Depending on the color changes to yellow, the results of this study were determined under wavelength of 450 nm to reveal the optical density (OD) via spectrophotometrically. The OD value is proportional to the amount of human IFN-γ in the sample of the healthy control and the cancer patients. The blood concentration of IFN-γ was substantially greater in women with breast cancer than in the control group (43.15 ± 4.68 pg/ml vs. 18.8 ± 3.89 pg/ml), respectively. Our results showed an elevation of Interferon-gamma (IFN-γ) in most of the serum samples from women with breast cancer. The conclusions of this study are the main findings, INF-y protein was employed as a marker and assistance in diagnosing breast cancer based on the results of the calculation of protein concentration from the ELISA test.
Keywords:
    Interferon gamma ELISA technique Breast cancer Serum concentration
Main Subjects:
  • Biotechnology
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(2021). Determination of Interferon Gamma Protein in Serum of Breast Cancer Patients Using the ELISA. Journal of Applied Sciences and Nanotechnology, 2(1), 37-48. doi: 10.53293/jasn.2021.4295.1089
Noor E. Hassan; Abbas A. Mohammed. "Determination of Interferon Gamma Protein in Serum of Breast Cancer Patients Using the ELISA". Journal of Applied Sciences and Nanotechnology, 2, 1, 2021, 37-48. doi: 10.53293/jasn.2021.4295.1089
(2021). 'Determination of Interferon Gamma Protein in Serum of Breast Cancer Patients Using the ELISA', Journal of Applied Sciences and Nanotechnology, 2(1), pp. 37-48. doi: 10.53293/jasn.2021.4295.1089
Determination of Interferon Gamma Protein in Serum of Breast Cancer Patients Using the ELISA. Journal of Applied Sciences and Nanotechnology, 2021; 2(1): 37-48. doi: 10.53293/jasn.2021.4295.1089
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Journal Information

Publisher: University of Technology - IRAQ

Email:  jasn@uotechnology.edu.iq

Editor-in-chief: Prof. Dr. Adawiya J. Haider

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Applied Sciences and Nanotechnology (JASN)

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