Biotechnology
Preparation and Characterization of Bi2Mo6 (M = Mo, W) for Antibacterial Activity

Huda N. Abid; Amar Al-Keisy; Duha S. Ahmed; Sangeeta Singh

Volume 3, Issue 2 , June 2023, , Page 32-40

https://doi.org/10.53293/jasn.2022.5250.1179

Abstract
  In recent years, Bi-based photocatalysts have begun to be applied in biological applications. However, the antibacterial ability of a Bi-based photocatalyst is still unclear. In this study, Bi2MoO6 and Bi2WO6 were successfully synthesized by a hydrothermal approach. The fabricated samples were characterized ...  Read More ...

Biotechnology
Antioxidant Properties of Galangin with β-cyclodextrin: An in Vitro and in Vivo

Zainab S. Abbas; Ghassan M. Sulaiman; Majid S. Jabir; Hamdoon A. Mohammed; Salman A. A. Mohammed

Volume 3, Issue 1 , March 2023, , Page 80-89

https://doi.org/10.53293/jasn.2022.4876.1157

Abstract
  Galangin, a non-toxic phytochemical, is known to have a variety of therapeutic uses. This study looked into the role of inclusion complexes of galangin/β-cyclodextrin in increasing antioxidant activity over pure galangin. The role of this inclusion complex in increasing antioxidant activity in comparison ...  Read More ...

Biotechnology
Biosynthesis and Optimization of PolyHydroxyalkanoate (PHA) Production by Acinetobacter lwoffii Isolate

Karrar K. Hameed; Iman H. Gatea; Amal A. Hussein

Volume 3, Issue 1 , March 2023, , Page 90-103

https://doi.org/10.53293/jasn.2022.4970.1167

Abstract
  Polyhydroxyalkanoate (PHA) is an eco-friendly polymer that has various important biomedical uses, as well as biodegradability, drug delivery, and medical implants. It is a kind of polyester synthesized by various microorganisms as energy reserve material under inappropriate conditions. This study focused ...  Read More ...

Biotechnology
Oncolytic NDV-AMHA1 Strain Effective Against Glioblastoma Cancer Cells

Zahraa A. Kadhim; Ghassan M. Sulaiman; Ahmed M. Al-Shammari; Hamdoon A. Mohammed; Salman A. A. Mohammed

Volume 3, Issue 1 , March 2023, , Page 124-136

https://doi.org/10.53293/jasn.2022.5016.1171

Abstract
  Glioblastoma multiforme (GBM) is one of the most life-threatening types of cancer that is difficult to treat. The search for effective yet safe therapy is progressing and non-conventional therapies such as using viruses as a smart and selective agent against cancer are promising. The aim of the study ...  Read More ...

Biotechnology
Study of the Physicochemical and Microbiological Properties of Mozzarella Cheese Coated with Edible Casings Subsidized with Green Tea

Ihab M. Abdulhadi; Mahdi Abbas Shakak; Kifah S. Doosh

Volume 3, Issue 1 , March 2023, , Page 151-163

https://doi.org/10.53293/jasn.2022.5086.1175

Abstract
  In this study, mozzarella cheese covered with edible and biodegradable films was manufactured from whey proteins reinforced with green alcoholic extract to preserve the environment from degradable plastics surrounding it. Two types of mozzarella cheese were manufactured. The first made unwrapped mozzarella ...  Read More ...

Biotechnology
Extraction and Purification of Pullulanase from Local Mushrooms

Dalal S. Bedan

Volume 3, Issue 1 , March 2023, , Page 164-173

https://doi.org/10.53293/jasn.2022.5123.1177

Abstract
  Pullulanase is defined as an extracellular carbohydrase enzyme responsible for the hydrolysis of pullulan into maltotriose. Pullulanase can be extracted from Agaricus bisporus edible mushroom and concentrated by dialysis tube using Tris-HCl buffer. This enzyme was purified by different ratios of ammonium ...  Read More ...

Biotechnology
Role of Microalgae in Environmental Biotechnology to Remove Heavy Metals

Muzhda Q. Qader; Yahya A. Shekha

Volume 3, Issue 1 , March 2023, , Page 174-184

https://doi.org/10.53293/jasn.2022.5346.1183

Abstract
  The objective of the study is to evaluate the role of microalgae in heavy metal remediation (Pb and Cd). Three microalgae species were used (Tetradesmus nygaardi, Scenedesmus quadricauda, and Coelastrella sp.) with four concentrations of both tested heavy metals (lead and cadmium) were, (5, 15, 35, and ...  Read More ...

Biotechnology
Study the Effect of Cinnamon and Tea Tree Oils on Biofilm Formation of Klebsiella Pneumoniae

Halah F. Rafeeq; Zahraa A. Sharba

Volume 2, Issue 2 , June 2022, , Page 16-26

https://doi.org/10.53293/jasn.2022.4246.1082

Abstract
  Klebsiella pneumoniae is a noteworthy human pathogen. As a virulence factor, these bacteria may create a thick coating of extracellular biofilm. This aids the organism's adhesion to biotic as well as abiotic surfaces, preventing antimicrobial agents from doing their job. Infections caused by bacterial ...  Read More ...

Biotechnology
DNA Analysis of qacE Gene in Pseudomonas Aeruginosa Isolated from Iraqi Patient

Rana M. Abdullah; Shahla N. Al-Azzawi

Volume 2, Issue 2 , June 2022, , Page 27-37

https://doi.org/10.53293/jasn.2022.4168.1077

Abstract
  Pseudomonas aeruginosa is an opportunistic pathogen that can cause different infections such as nosocomial bacterial infection, Urinary tract infection, Meningitis, Eye infection, Otitis Media, Respiratory infections, and Pneumonia, especially in people with Cystic fibrosis, Bone and joints infection, ...  Read More ...

Biotechnology
Computational Analysis of Interleukin 17A Activity in Breast Cancer Patients Using Bioinformatics Methods

Abdalrahman S. Mohammed‬; Abbas A. Al-Janabi

Volume 2, Issue 2 , June 2022, , Page 79-85

https://doi.org/10.53293/jasn.2021.3878.1052

Abstract
  In the current study, bioinformatics approach has been utilized to discover the sequences and structures analysis of IL-17A of breast cancer and compares with normal sequence from NCBI. The current study was aimed to discuss the possibility of using IL-17A as a marker for patients with breast cancer. ...  Read More ...

Biotechnology
Determination of Risk Factors and Some Biomarkers Parameters during Infected Iraqis with Covid-19

Rafah A. Salih; Ali A. Taha; Nadira S. Mohamed

Volume 2, Issue 2 , June 2022, , Page 112-123

https://doi.org/10.53293/jasn.2021.4112.1071

Abstract
  Covid-19 is a global challenge that drives health care to its limits. Biological parameters associated with increased risk of severe or fatal disease courses were identified, including conditions connected with a poor state of health, organ damage, and coagulation dysfunction. In a recent study, risk ...  Read More ...

Biotechnology
Using Bioinformatics and NCBI Tools to Sequence and Structure Analysis of Transcription Factor 7 Like 2 Gene (TCF7L2) in Iraqi Diabetic Mellitus Type II Patients

Batool K. Queen; Abbas A. Mohammed

Volume 2, Issue 1 , March 2022, , Page 1-10

https://doi.org/10.53293/jasn.2021.4198.1079

Abstract
  In this paper, the study aimed to analyze the sequence and structure of Transcription Factor 7 Like 2 gene (TCF7L2) in Iraqi Diabetic Mellitus Type II (T2DM) Patients and was compared with standard sequence form National Center of Bioinformatics Tools (NCBI) using BLAST. Ten blood samples of (T2DM) Iraqi ...  Read More ...

Biotechnology
Determination of Interferon Gamma Protein in Serum of Breast Cancer Patients Using the ELISA

Noor E. Hassan; Abbas A. Mohammed

Volume 2, Issue 1 , March 2022, , Page 37-48

https://doi.org/10.53293/jasn.2021.4295.1089

Abstract
  Interferon gamma (IFN-γ) is a cytokine involved in the induction and modulation of various immune responses. INF-γ has recently been associated with breast cancer and the stage of the disease. Also, it can be used as a cancer marker in determining disease activity. The ELISA test is a technique ...  Read More ...

Biotechnology
The Biological Activity of Mycosynthesized Silver Nanoparticles Against some Pathogenic Bacteria

Rana A. Hikmet; Nehia N. Hussein

Volume 2, Issue 1 , March 2022, , Page 59-68

https://doi.org/10.53293/jasn.2021.3963.1063

Abstract
  This study was carried out for the mycosynthesis of silver nanoparticles by Candida albicans supernatant.  All the isolates used in this study were taken from the patients who existed at Al-Elweya children's teaching hospital in Baghdad, Iraq. Mycosynthesized silver nanoparticles were characterized ...  Read More ...

Biotechnology
Effects of Melatonin and Flaxseeds Oil on Oxidative Stress and Inflammatory Parameters in Type 2 Diabetic Patients

Haitham Kadhim; Arwa Al-Shuwiakh; Noor M. Ali; Faruk H. Aljawad; Hashim M. Hashim; Ashwaq Q. Gorji

Volume 2, Issue 1 , March 2022, , Page 90-98

https://doi.org/10.53293/jasn.2021.4049.1068

Abstract
  In patients with diabetes, chronic inflammation is characterised by the increase in C-reactive protein (CRP) and tumour necrosis alpha (TNF-α). The consumption of melatonin and flaxseed oil may improve insulin sensitivity and the effectiveness of diabetic medications. The current study investigated ...  Read More ...

Biotechnology
Evidence of Antioxidant Activity of Novel L-Glutaminase Purified from L. Gasseri BRLHM

Butheina Hasson; Likaa Mahdi; Rajwa Essa

Volume 1, Issue 4 , December 2021, , Page 44-51

https://doi.org/10.53293/jasn.2021.3969.1064

Abstract
  Probiotic strains have the potential to be used as bio-preservatives and functional radical scavenging treatments in the future.  Antioxidant tests, including DPPH radical scavenging, were used to evaluate the antioxidant effects of extracellular L- Glutaminase isolated from L. gasseri BRLHM. Parameters ...  Read More ...

Biotechnology
Obtaining L - Glutamate Oxidase Enzyme from Streptomyces and Estimation Its Toxicity on Blood Components

Dina H. Abdul Hameed; Entesar H. Ali

Volume 1, Issue 4 , December 2021, , Page 61-68

https://doi.org/10.53293/jasn.2021.4042.1069

Abstract
  Streptomyces was isolated from the soil and then tested to diagnose morphologically and microscopically. Also, cultivated in the production medium of the L-glutamate oxidase enzyme, for which the wheat bran culture media was used, and then the extracellular L-glutamate oxidase enzyme was extracted using ...  Read More ...

Biotechnology
Antioxidant Activities of Resveratrol Loaded Poloxamer 407: An In Vitro and In Vivo Study

Ali A. Al-Jubori; Ghassan M. Sulaiman; Amer T. Tawfeeq

Volume 1, Issue 3 , September 2021, , Page 1-12

https://doi.org/10.53293/jasn.2021.3809.1046

Abstract
  Resveratrol (RES) is a bioactive molecule with potent antioxidant properties, and it constitutes an essential component of herbal medicine. This study was designed to use a nano-syntheses approach to encapsulate RES in Poloxamer 407 nanoparticles. This type of nano-construction has been employed in a ...  Read More ...

Biotechnology
The Effect of LRP-1 Gene and Mir-205 on Cardiovascular Disease in Iraqi Patients with Familial Hypercholesterolemia

Zinah A. Ghareeb; Hiba Abdel-Hassan AlKhafaji; Mohanad K. Aneed Al-Saedi

Volume 1, Issue 3 , September 2021, , Page 24-31

https://doi.org/10.53293/jasn.2021.3691.1036

Abstract
  Familial hypercholesterolemia (FH) is a genetic disorder characterized by autosomal inheritance in genes related to LDL-C metabolism, with the major clinical features of hyper-LDL-cholesterolemia and premature coronary artery disease. (LRP-1) is a member of the LDLR family. It is a membrane receptor ...  Read More ...

Biotechnology
Primer and Probe Designing to Detect SNP rs 4073 in Interleukin-8 Gene in Iraqi Patients with Bronchial Asthma

Media F. Ali Jan; Basima Q. Hassan Al-Saadi; Hiba M. Abdel-Hassan Al-Khafaji; Mohanad K. Aneed Al-Saedi

Volume 1, Issue 3 , September 2021, , Page 51-57

https://doi.org/10.53293/jasn.2021.3542.1034

Abstract
  Asthma is one of the most prevalent non-communicable illnesses, and it has a significant influence on the quality of life for many people. Asthma is now thought to be a multifaceted condition, with interactions between genetic susceptibility, host factors, and environmental exposures increasingly being ...  Read More ...

Biotechnology
Correlation of MicroRNAs-122a Gene Expression with Diabetic for Iraqi Patients

Noor Younus; Maha Altaee; Zahraa Abdul-Munim Sharba

Volume 1, Issue 3 , September 2021, , Page 64-72

https://doi.org/10.53293/jasn.2021.3789.1043

Abstract
  This study was carried out to describe the gene expression of the micro RNA 122a gene with the development of diabetes in Iraq. The difference in gene expression between patients and healthy controls was properly considered. In this study, blood was isolated from 121 individuals divided into two groups ...  Read More ...

Biotechnology
Evaluation The Antibacterial Activity of Biosynthesis Silver Nanoparticles by Lactobacillus Gasseri Bacteria

Raghad Jabbar; Nehia N. Hussein

Volume 1, Issue 3 , September 2021, , Page 86-95

https://doi.org/10.53293/jasn.2021.3898.1057

Abstract
  Biosynthesis of AgNPs is a new approach in the field of nanotechnology with optimistic implementation in medicine, food control, and pharmacology. In this study, the silver nanoparticles were produced by Lactobacillus gasseri filtrate. The production of AgNPs was confirmed by the color change from yellow ...  Read More ...

Biotechnology
Characterization of Treated Multi-Walled Carbon Nanotubes and Antibacterial Properties

Noor Ali; Ali Taha; Duha S. Ahmed

Volume 1, Issue 2 , June 2021, , Page 1-9

https://doi.org/10.53293/jasn.2021.11636

Abstract
  In the present study, the synthesized Multi-Walled Carbon Nanotubes (MWCNTs) were chemically treated with a mixture of acids to produce functionalized MWNTs. The functionalized MWCNTs were characterized by X-Ray Diffraction Analysis (XRD), Zeta potential and Field-Emission Scanning Electron Microscopy ...  Read More ...

Biotechnology
Enhanced Production of Fibrinolytic Enzyme from Pseudomonas aeruginosa by Optimization Media Components

Bilal Jasim; Entesar Ali

Volume 1, Issue 2 , June 2021, , Page 58-65

https://doi.org/10.53293/jasn.2021.11637

Abstract
  Fibrinolytic enzyme is factor that lysis fibrin clots. This fibrinolytic factor has prospective use to treat cardiovascular diseases, such as stroke and heart attack. Cardiovascular diseases attracted worldwide attention for their elevation morbidity and mortality. Expensive cost and fatally undesired ...  Read More ...

Biotechnology
Evaluation of the Biosynthesized Silver Nanoparticles’’ Effects on Biofilm Formation

Nehia Hussein; Mina M. Khadum

Volume 1, Issue 1 , April 2021, , Page 23-31

https://doi.org/10.53293/jasn.2021.11019

Abstract
  In this study 50 isolates were obtained from the Baghdad teaching city medicine laboratories, from wounds and burns. Isolates were identified exercise VITEK 2 system (Biomerieux). Streptococcus pyogenes isolate was used to create the biosynthesize of silver nanoparticles’’ against some pathogenic ...  Read More ...